You are here

Precise montaging and metric quantification of retinal surface area from ultra-widefield fundus photography and fluorescein angiography

TitlePrecise montaging and metric quantification of retinal surface area from ultra-widefield fundus photography and fluorescein angiography
Publication TypeJournal Article
Year of Publication2014
AuthorsCroft, DE, van Hemert, J, Wykoff, CC, Clifton, D, Verhoek, M, Fleming, A, Brown, DM
Journal TitleOphthalmic Surg Lasers Imaging Retina
Volume45
Pages312–317
Journal DateJul
ISSN2325-8179 (Electronic)
Keywordsmedical; retinal imaging
Abstract

BACKGROUND AND OBJECTIVE: Accurate quantification of retinal surface area from ultra-widefield (UWF) images is challenging due to warping produced when the retina is projected onto a two-dimensional plane for analysis. By accounting for this, the authors sought to precisely montage and accurately quantify retinal surface area in square millimeters. PATIENTS AND METHODS: Montages were created using Optos 200Tx (Optos, Dunfermline, U.K.) images taken at different gaze angles. A transformation projected the images to their correct location on a three-dimensional model. Area was quantified with spherical trigonometry. Warping, precision, and accuracy were assessed. RESULTS: Uncorrected, posterior pixels represented up to 79% greater surface area than peripheral pixels. Assessing precision, a standard region was quantified across 10 montages of the same eye (RSD: 0.7%; mean: 408.97 mm(2); range: 405.34-413.87 mm(2)). Assessing accuracy, 50 patients' disc areas were quantified (mean: 2.21 mm(2); SE: 0.06 mm(2)), and the results fell within the normative range. CONCLUSION: By accounting for warping inherent in UWF images, precise montaging and accurate quantification of retinal surface area in square millimeters were achieved. [Ophthalmic Surg Lasers Imaging Retina. 2014;45:312-317.].

DOI10.3928/23258160-20140709-07